| Drury University | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |
| Lewis University | NCAA D2 | Great Lakes Valley Conference | IL | Men & Women | |
| Lincoln University (Missouri) | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |
| Maryville University of Saint Louis | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |
| McKendree University | NCAA D2 | Great Lakes Valley Conference | IL | Men & Women | |
| Missouri University of Science and Technology | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |
| Quincy University | NCAA D2 | Great Lakes Valley Conference | IL | Men & Women | |
| Rockhurst University | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |
| Southwest Baptist University | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |
| Truman State University | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |
| University of Illinois Springfield | NCAA D2 | Great Lakes Valley Conference | IL | Men & Women | |
| University of Indianapolis | NCAA D2 | Great Lakes Valley Conference | IN | Men & Women | |
| Upper Iowa University | NCAA D2 | Great Lakes Valley Conference | IA | Women | |
| William Jewell College | NCAA D2 | Great Lakes Valley Conference | MO | Men & Women | |